Developing High-Performance Proteins with Industry-Leading Gene Evolution Technologies
Enzymes and other proteins are naturally occurring, complex molecules made up of long chains of individually linked amino acids. Found everywhere in nature are 20 different amino acids, each having distinctive chemical properties. Protein amino acid sequences are directly translated from a gene where DNA acts like a blueprint. The resulting unique sequence of amino acids of each protein directs it to take on a specific form and function. A change in just one amino acid can greatly influence the function and properties of a protein such as an enzyme or an antibody. As you might imagine, enabling technologies are needed to harness the potential compounding diversity of 20 possibilities at each position in a protein sequence which typically measures hundreds of amino acids in length.
State-of-the-art gene evolution services
Verenium possesses patented, state-of-the-art gene evolution services that make possible optimization of proteins at the DNA level. Verenium's suite of DirectEvolution® technologies provides significant competitive advantages, including the most comprehensive and non-biased gene evolution platform, the ability to make fine changes across an entire gene and the freedom to reassemble the widest variety of genes with ultimate precision. Our patented technologies enable the modification of gene sequences to achieve not only increased protein activity and stability, but also other desirable qualities such as increased expression for enzyme product manufacturing without changing the fundamental amino acid sequence.
Two complementary methods lie beneath Verenium's DirectEvolution® platform:
Gene Site Saturation MutagenesisSM (GSSMSM) Technology
Verenium's patented GSSMSM technology rapidly generates protein variants by incorporating any or all of the 20 possible amino acids at every position along a protein's sequence allowing all possibilities to be tested in an unbiased manner. The library of variants created using GSSMSM technology is then available to be expressed and screened for improved properties. From our experience, often just a few amino acid changes can result in proteins with significantly improved characteristics such as temperature stability, pH stability, increased reaction rate or resistance to deactivating chemicals.
Tunable GeneReassemblySM (TGRSM ) Technology
Verenium uses Tunable GeneReassemblySM technology to optimize the characteristics of proteins by combining the best properties of candidate genes into a new, high performance molecule. Flexible but exacting design is at the heart of TGRSM which, in addition to sequence information, can incorporate supplementary knowledge such as 3-D structural information and codon optimization. GeneReassemblySM technology allows blending of gene sequences at precise positions, enabling us to create complete combinatorial libraries of all favorable variants from GSSMSM as well as reassembled genes from the most useful members of an entire protein family. As such, TGRSM technology is an excellent complement to GSSMSM and significantly increases the success of finding gene products that will lead to novel and next generation enzymes.